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Isotopically labelled internal standard
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Multilevel calibrators
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Online sample preparation
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Upgrade available for the dermination of 3-epi-25-OH-Vitamin D3/D2
Vitamin D has been widely recognised as a biomarker in relation to osteoporosis and osteopenia. It has also been shown to play a prominent role in immune response, cardiovascular disease and certain types of cancer. Vitamin D analysis, therefore, carries significance in several respects.
25-OH-vitamin D3 constitutes the principal diagnostic target and is the main metabolite of vitamin D in humans; it is the precursor of the physiologically most active compound 1,25-(OH)2-Vitamin D3. Vitamin D deficiency is treated with vitamin D3 or vitamin D2 supplementation. Vitamin D2 is easier to produce synthetically and, where prescribed, its monitoring is also necessary.
This reagent kit allows for the specific analysis of 25-OH-vitamin D3 and 25-OH-vitamin D2 in serum and plasma by tandem mass spectrometry. Manual sample preparation is minimised and limited to simple and effective protein precipitation. A subsequent online trap column concentrates the analytes and separates interfering substances. A six-port-valve connects the trap column to an HPLC column which provides further purification. The use of atmospheric pressure chemical ionisation (APCI) and an isotopically labelled internal standard assures precision and robustness and minimises ion suppression effects. The 3PLUS1® multilevel calibrators offer additional precision.
The upgrade to MassChrom® 25-OH-vitamin D3/D2 analysis allows for the separation of the main metabolites of vitamins D3 and D2, 25-Hydroxycholecalciferol and 25-Hydroxyergocalciferol, from their epimeric forms 3-epi-25-OH-vitamin D3 and 3-epi-25-OH-vitamin D2, hence allowing for separate quantification by LC MS/MS.
| Order no. | Product | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 62000 |
MassChrom® Reagent kit for the LC-MS/MS analysis of 25-OH-Vitamin D3 and D2 in serum/plasma For 200 analyses |
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| 62000/1000 |
MassChrom® Integrated kit for the LC-MS/MS analysis of 25-OH-Vitamin D3 and D2 in serum/plasma, For 1000 analyses, complete with: Analytical column Trap-column 3PLUS1® Calibrators MassCheck® Controls |
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| 62000/1000/F |
MassChrom® Integrated kit for the LC-MS/MS analysis of 25-OH-Vitamin D3 and D2 in serum/plasma, For the automated sample preparation with 96 well filter plates. For 1000 analyses, complete with: Analytical column Trap-column 3PLUS1® Calibrators MassCheck® Controls |
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| Components available separately: | ||||||||||
| 62001 | Mobile Phase A, 1000 ml | |||||||||
| 62002 | Mobile Phase B, 1000 ml | |||||||||
| 62009 | Rinsing Solution, 1000 ml | |||||||||
| 62003 | Precipitation Reagent, 5 ml | |||||||||
| 62004 | Internal Standard, 40 ml | |||||||||
| 3006 | Reaction vials, 100 pcs./td> | |||||||||
| Multilevel Calibrators and Startup Accessories: | ||||||||||
| 62028 | 3PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2, 4 x 1 ml | |||||||||
| 62039 | 6PLUS1® Multilevel Serum Calibrator Set 25-OH-Vitamin D3/D2, 7 x 1 ml | |||||||||
| 62110 | Trap column, 1 pc. | |||||||||
| 62100 | Analytical column, 1 pc. | |||||||||
| 62015 | Tuning Mix 25-OH-Vitamin D3/D2, 2 ml | |||||||||
| 15010 | PEEK prefilter housing, 1 pc. | |||||||||
| 15011 | PEEK-encased Prefilter, 2 μm, 5 pcs. | |||||||||
| Chromsystems MassCheck® Controls (lyoph.): | ||||||||||
| 0221 | MassCheck® 25-OH-Vitamin D3/D2 Serum Control, Bi-Level (I+II), 2 x 5 x 1 ml | |||||||||
| 0222 | MassCheck® 25-OH-Vitamin D3/D2 Serum Control, Level I, 5 x 1 ml | |||||||||
| 0223 | MassCheck® 25-OH-Vitamin D3/D2 Serum Control, Level II, 5 x 1 ml | |||||||||
| 0256 | MassCheck® 25-OH-Vitamin D3/D2 Serum Control, Level III, 5 x 1 ml | |||||||||
| Accessories for sample preparation with 96 well filter plates: | ||||||||||
| 62057 | 96 Well filter plates, 3 pcs. | |||||||||
| 62058 | Collection plates, 3 pcs. | |||||||||
| 62059 | Pierceable adhesive seals, 3 pcs. | |||||||||
| Upgrade accessories for the analysis of diastereomeric forms of 25-OH-Vitamin D3/D2: | ||||||||||
| 62044 | Internal Standard Mix (for 200 analyses), 40 ml | |||||||||
| 62029 | 3PLUS1® Multilevel Serum Calibrator Set 3-epi-25-OH-D3/D2 and 25-OH-D3/D2 (lyoph.), 4 x 1 ml | |||||||||
| 0310 | MassCheck® 3-epi-25-OH-D3/D2 and 25-OH- D3/D2 Serum Control, Bi-Level (I + II) (lyoph.), 2 x 5 x 1 ml | |||||||||
| 0311 | MassCheck® 3-epi-25-OH-D3/D2 and 25-OH- D3/D2 Serum Control, Level I (lyoph.), 5 x 1 ml | |||||||||
| 0312 | MassCheck® 3-epi-25-OH-D3/D2 and 25-OH-D3/D2 Serum Control, Level II (lyoph.), 5 x 1 ml | |||||||||
| 62120 | Analytical column for determination of epimers (equilibrated with test chromatogram), 1 pc. | |||||||||
| 62110/Epi | Trap column for determination of epimers (equilibrated with test chromatogram), 1 pc. | |||||||||
| 62016 |
Tuning Mix, contains 25-OH-Vitamin D3/D2 and ISTD 1, 1 m
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